Journal: Cell death & disease

Article Title: Genetic deletion of G protein-coupled receptor 56 aggravates traumatic brain injury through the microglial CCL3/4/5 upregulation targeted to CCR5.

doi: 10.1038/s41419-025-07501-7

Figure Lengend Snippet: Fig. 4 Deleting Gpr56 regulates the release of chemokines from immune cells in mice three days following TBI. A The working model of Luminex assay. B Heat map from all Bio-Plex Pro Mouse Chemokine Panel data features 21 magnetic bead-based immunoassays in experimental groups. C Quantitative analysis showed significantly increased chemokines in the brains of Gpr56 KO mice compared to WT controls three days after TBI. n = 5 mice per group. One-way analysis of variance followed by Tukey’s post hoc test was used. All data are shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 versus Sham+WT group. #p < 0.05, ##p < 0.01, ###p < 0.001 versus TBI+Gpr56 KO group.

Article Snippet: Cell Death and Disease (2025) 16:175 Deletion of Gpr56 regulates the release of related chemokines from immune cells in mice three days following TBI Luminex assay based on the principles of microspheres and flow cytometry (Fig. 4A) was used to estimate the protein expression levels of cytokines/chemokines, which were detected by the BioPlex Pro Mouse Cytokine Panel featuring 31 magnetic bead-based immunoassays.

Techniques: Luminex

Journal: Cell death & disease

Article Title: Genetic deletion of G protein-coupled receptor 56 aggravates traumatic brain injury through the microglial CCL3/4/5 upregulation targeted to CCR5.

doi: 10.1038/s41419-025-07501-7

Figure Lengend Snippet: Fig. 5 Gpr56 deletion promotes peripheral immune cell infiltration in mice three days following TBI. A Flow cytometry examination of circulating immune cells in the brain three days after TBI. B Quantification of immune cells that have infiltrated the brain. n = 6 mice per group. C Representative immunofluorescence staining images of Iba-1 in the ipsilateral cerebral cortex three days following TBI. Scale bars: 100 µm. D Quantification of the Iba-1 area. n = 6 mice per group. One-way analysis of variance followed by Tukey’s post hoc test was used. All data are shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Cell Death and Disease (2025) 16:175 Deletion of Gpr56 regulates the release of related chemokines from immune cells in mice three days following TBI Luminex assay based on the principles of microspheres and flow cytometry (Fig. 4A) was used to estimate the protein expression levels of cytokines/chemokines, which were detected by the BioPlex Pro Mouse Cytokine Panel featuring 31 magnetic bead-based immunoassays.

Techniques: Flow Cytometry, Staining

Journal: Cell death & disease

Article Title: Genetic deletion of G protein-coupled receptor 56 aggravates traumatic brain injury through the microglial CCL3/4/5 upregulation targeted to CCR5.

doi: 10.1038/s41419-025-07501-7

Figure Lengend Snippet: Fig. 6 GPR56 expression levels were reduced in microglia after TBI. A Representative Western blots of GPR56. B Quantification of relative protein expression normalized to the optical density of β-actin. C GPR56 expression analysis of the GSE52564 dataset in different brain cell types. D GPR56 expression analysis with the GSE167459 dataset in astrocytes and microglia between Sham and TBI group. E Isolation of astrocytes and microglia by Flow cytometry. F Changes in mRNA expression levels of GPR56 in astrocyte three days following TBI. G Changes in mRNA expression levels of GPR56 in microglial three days following TBI. n = 5 mice per group. All data are shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Cell Death and Disease (2025) 16:175 Deletion of Gpr56 regulates the release of related chemokines from immune cells in mice three days following TBI Luminex assay based on the principles of microspheres and flow cytometry (Fig. 4A) was used to estimate the protein expression levels of cytokines/chemokines, which were detected by the BioPlex Pro Mouse Cytokine Panel featuring 31 magnetic bead-based immunoassays.

Techniques: Expressing, Western Blot, Isolation, Flow Cytometry

Journal: Biology of Sex Differences

Article Title: Sex differences in disease severity and immune responses in murine and human inflammatory arthritis

doi: 10.1186/s13293-026-00840-w

Figure Lengend Snippet: Upregulated cytokine/chemokines profiles show a male bias in serum and female bias in joint tissues. On day 29 after the first CII challenge, mice were euthanized. Serum and joint tissue lysates were collected. A panel of 44 cytokines and chemokines were evaluated in the serum and joint tissue lysate samples using mouse cytokine/chemokine 44-plex discovery assay array (MD44). The volcano plot analysis of (A) serum and (C) joint tissue was performed with Log 2 fold-change (CIA-Saline) values. The red dots indicate higher in females, blue dots indicate higher in males and gray dots indicate unchanged values. The heatmap analysis performed with Log 2 fold-change (CIA-Saline) values on the selected (based on the volcano plot analysis) cytokine/chemokine in (B) serum (D) joint tissue showed the differential abundance of cytokine and chemokines in male and female mice. Cytokine and chemokine profiles of CIA mice were represented as Log 2 values normalized to respective saline group with spread across the scale of − 1.5 to + 1.5-fold (blue to red). N = 5 mice per group. One of two independent experiments

Article Snippet: A panel of 44 cytokines and chemokines were quantified in serum and joint tissue lysates using the Mouse Cytokine/Chemokine 44-Plex Discovery Assay ® Array (MD44), a Luminex-based assay platform (Eve Technologies Corporation, Calgary, AB).

Techniques: Saline